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gltT [2019-01-08 10:36:26]
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gltT [2019-01-08 10:36:26]

major high-affinity Na+-coupled glutamate/ aspartate symport protein
Locus
BSU10220
Isoelectric point
9.03
Molecular weight
45.76 kDa
Protein length
429 aa Sequence Blast
Gene length
1287 bp Sequence Blast
Function
glutamate and aspartate uptake
Product
major Na+-coupled glutamate/ aspartate symport protein
Essential
no
Synonyms
yhfG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → Solute:sodium symporter family
  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → Dicarboxylate/amino acid:cation symporter
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of glutamine/ glutamate
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,096,560 → 1,097,849

    Phenotypes of a mutant

  • impaired uptake of glutamate and aspartate PubMed

    • The protein

    Protein family

  • dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family (according to UniProt)

    • Paralogous protein(s)

  • GltP, DctP

    • Kinetic information

  • Km values: 37 ± 5 μM for glutamate, 41 ± 9 μM for aspartate PubMed

    • Effectors of protein activity

  • aspartate uptake is competitively inhibited by glutamate PubMed

    • Structure

  • 4IZM (the protein from Pyrococcus horikoshii, 35% identity, 73% similarity) PubMed
  • 4KY0 (the glutamate transporter of Thermococcus kodakarensis, 35% identity, 72% similarity) PubMed

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    gltT
    Description
    PubMed
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    Biological materials

    Mutant

  • BP233 (ΔgltT::spc), available in Fabian Commichau's lab
  • BP235 (ΔgltT::spc ΔgltP::cat), available in Fabian Commichau's lab
  • GP2247 (ΔgltT::ermC), available in Jörg Stülke's lab
  • GP2248 (ΔgltT::aphA3), available in Jörg Stülke's lab
  • GP2722 (ΔgltT::zeo), available in Jörg Stülke's lab
  • BKE10220 (ΔgltT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT
  • BKK10220 (ΔgltT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

    • Expression vectors

  • for expression in B. subtilis, in pBQ200: pBP56, available in Fabian Commichau's lab

    • References

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